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Bibliography Tag: glyphosate

Suppa et al., 2020

Suppa, A., Kvist, J., Li, X., Dhandapani, V., Almulla, H., Tian, A. Y., Kissane, S., Zhou, J., Perotti, A., Mangelson, H., Langford, K., Rossi, V., Brown, J. B., & Orsini, L.; “Roundup causes embryonic development failure and alters metabolic pathways and gut microbiota functionality in non-target species;” Microbiome, 2020, 8(1), 170; DOI: 10.1186/s40168-020-00943-5.


BACKGROUND: Research around the weedkiller Roundup is among the most contentious of the twenty-first century. Scientists have provided inconclusive evidence that the weedkiller causes cancer and other life-threatening diseases, while industry-paid research reports that the weedkiller has no adverse effect on humans or animals. Much of the controversial evidence on Roundup is rooted in the approach used to determine safe use of chemicals, defined by outdated toxicity tests. We apply a system biology approach to the biomedical and ecological model species Daphnia to quantify the impact of glyphosate and of its commercial formula, Roundup, on fitness, genome-wide transcription and gut microbiota, taking full advantage of clonal reproduction in Daphnia. We then apply machine learning-based statistical analysis to identify and prioritize correlations between genome-wide transcriptional and microbiota changes.

RESULTS: We demonstrate that chronic exposure to ecologically relevant concentrations of glyphosate and Roundup at the approved regulatory threshold for drinking water in the US induce embryonic developmental failure, induce significant DNA damage (genotoxicity), and interfere with signaling. Furthermore, chronic exposure to the weedkiller alters the gut microbiota functionality and composition interfering with carbon and fat metabolism, as well as homeostasis. Using the “Reactome,” we identify conserved pathways across the Tree of Life, which are potential targets for Roundup in other species, including liver metabolism, inflammation pathways, and collagen degradation, responsible for the repair of wounds and tissue remodeling.

CONCLUSIONS: Our results show that chronic exposure to concentrations of Roundup and glyphosate at the approved regulatory threshold for drinking water causes embryonic development failure and alteration of key metabolic functions via direct effect on the host molecular processes and indirect effect on the gut microbiota. The ecological model species Daphnia occupies a central position in the food web of aquatic ecosystems, being the preferred food of small vertebrates and invertebrates as well as a grazer of algae and bacteria. The impact of the weedkiller on this keystone species has cascading effects on aquatic food webs, affecting their ability to deliver critical ecosystem services. FULL TEXT

Santos et al., 2020

Santos, J. S., Pontes, M. S., Santiago, E. F., Fiorucci, A. R., & Arruda, G. J.; “An efficient and simple method using a graphite oxide electrochemical sensor for the determination of glyphosate in environmental samples;” Science of The Total Environment, 2020, 749, 142385; DOI: 10.1016/j.scitotenv.2020.142385.


Excessive and indiscriminate use of the herbicide glyphosate (GLY) leaves the environment susceptible to its contamination. This work describes the development of a simple, inexpensive, and efficient electroanalytical method using graphite oxide paste electrode (GrO-PE) for the direct determination of GLY traces in groundwater samples, soybean extracts, and lettuce extracts. Under optimal experimental conditions, the developed sensor exhibited a linear response of the peak current intensity vs. the concentration, in the range of 1.8 x 10(-5) to 1.2 x 10(-3) mol L(-1) for GLY. The limits of detection and quantification are 1.7 x 10(-8) mol L(-1) and 5.6 x 10(-8) mol L(-1), respectively. The methodology developed here demonstrated a strong analytical performance, with high reproducibility, repeatability, and precision. Moreover, it successfully avoided interference from other substances, showing high selectivity. The GrO-PE sensor was effectively applied to determine GLY traces in real samples with recovery rates ranging from 98% to 102%. Results showed that the GrO-PE is effective and useful for GLY detection, with the advantage of not involving laborious modifications and complicated handling, making it a promising tool for environmental analysis. FULL TEXT

Rydz et al., 2020

Rydz, C. E., Larsen, K., & Peters, C. E.; “Estimating Exposure to Three Commonly Used, Potentially Carcinogenic Pesticides (Chlorolathonil, 2,4-D, and Glyphosate) Among Agricultural Workers in Canada;” Annals of Work Exposures and Health, 2020; DOI: 10.1093/annweh/wxaa109.


OBJECTIVES: Certain pesticides have been associated with adverse health outcomes including cancer and reproductive harms. However, little is known about the prevalence of occupational pesticide exposure among agricultural workers in Canada. The purpose of this study was to estimate the prevalence and likelihood of occupational exposure to pesticides in Canada’s agricultural industry, using three commonly used, potentially carcinogenic pesticides [chlorothalonil, 2,4-dichlorophenoxyacetic acid (2,4-D), and glyphosate] as an example.

METHODS: Estimates were calculated using the Canadian Census of Population and the Census of Agriculture. The number of workers and the proportion of farms applying ‘herbicides’ or ‘fungicides’ by farm type was estimated using survey data from the Census of Agriculture. These values were multiplied to yield the potential number of workers at risk of exposure. Likelihood of exposure (i.e. exposed, probably exposed, and possibly exposed) was then qualitatively assigned using information on crop type, primary expected tasks, crop production practices, and residue transfer data. Additional agricultural workers who are at risk of exposure but not captured by the Census of Agriculture were identified using the 2016 Census of Population.

RESULTS: An estimated range of 37 700-55 800 workers (11-13% of agricultural workers) were exposed to glyphosate in Canada while 30 800-43 600 workers (9-11%) and 9000-14 100 (2.9-3.2%) were exposed to 2,4-D and chlorothalonil, respectively. Approximately 70-75% of workers at risk of exposure were considered probably or possibly exposed to any of the pesticides. Glyphosate exposure was most common among workers in oilseed (29% of oilseed farm workers exposed) and dry pea/bean farms (28%), along with those providing support activities for farms (31%). 2,4-D exposure was most common in corn (28%), other grain (28%), and soybean farms (27%), while chlorothalonil exposure was more likely among greenhouse, nursery, and floriculture workers (42%), workers on farms (28%, for occupations not captured by the Census of Agriculture, specifically), and those providing support activities for farms (20%). Regional variations broadly reflected differences in farm types by province.

CONCLUSIONS: This study estimated the prevalence of occupational exposure to three pesticides in Canada. Seasonal and temporary agricultural workers, which were captured by the Census of Agriculture, contributed to many additionally exposed workers. A large percent of the workers who were considered at risk of exposure were considered probably or possibly exposed, indicating a need for enhanced data collection and availability on pesticide use data in Canada. The study’s methods can be applied to estimate workers’ exposures to other pesticides within the agricultural industry.

Masood et al., 2021

Masood, M. I., Naseem, M., Warda, S. A., Tapia-Laliena, M. A., Rehman, H. U., Nasim, M. J., & Schafer, K. H.; “Environment permissible concentrations of glyphosate in drinking water can influence the fate of neural stem cells from the subventricular zone of the postnatal mouse;” Environmental Pollution, 2021, 270, 116179; DOI: 10.1016/j.envpol.2020.116179.


The developing nervous system is highly vulnerable to environmental toxicants especially pesticides. Glyphosate pesticide induces neurotoxicity both in humans and rodents, but so far only when exposed to higher concentrations. A few studies, however, have also reported the risk of general toxicity of glyphosate at concentrations comparable to allowable limits set up by environmental protection authorities. In vitro data regarding glyphosate neurotoxicity at concentrations comparable to maximum permissible concentrations in drinking water is lacking. In the present study, we established an in vitro assay based upon neural stem cells (NSCs) from the subventricular zone of the postnatal mouse to decipher the effects of two maximum permissible concentrations of glyphosate in drinking water on the basic neurogenesis processes. Our results demonstrated that maximum permissible concentrations of glyphosate recognized by environmental protection authorities significantly reduced the cell migration and differentiation of NSCs as demonstrated by the downregulation of the expression levels of the neuronal ss-tubulin III and the astrocytic S100B genes. The expression of the cytoprotective gene CYP1A1 was downregulated whilst the expression of oxidative stresses indicator gene SOD1 was upregulated. The concentration comparable to non-toxic human plasma concentration significantly induced cytotoxicity and activated Ca(2+) signalling in the differentiated culture. Our findings demonstrated that the permissible concentrations of glyphosate in drinking water recognized by environmental protection authorities are capable of inducing neurotoxicity in the developing nervous system. FULL TEXT

Lopez-Castanos et al., 2020

Lopez-Castanos, K. A., Ortiz-Frade, L. A., Mendez, E., Quiroga-Gonzalez, E., Gonzalez-Fuentes, M. A., & Mendez-Albores, A.; “Indirect Quantification of Glyphosate by SERS Using an Incubation Process With Hemin as the Reporter Molecule: A Contribution to Signal Amplification Mechanism;” Frontiers in Chemistry, 2020, 8, 612076; DOI: 10.3389/fchem.2020.612076.


The indirect determination of the most used herbicide worldwide, glyphosate, was achieved by the SERS technique using hemin chloride as the reporter molecule. An incubation process between hemin and glyphosate solutions was required to obtain a reproducible Raman signal on SERS substrates consisting of silicon decorated with Ag nanoparticles (Si-AgNPs). At 780 nm of excitation wavelength, SERS spectra from hemin solutions do not show extra bands in the presence of glyphosate. However, the hemin bands increase in intensity as a function of glyphosate concentration. This allows the quantification of the herbicide using as marker band the signal associated with the ring breathing mode of pyridine at 745 cm-1. The linear range was from 1 × 10-10 to 1 × 10-5 M and the limit of detection (LOD) was 9.59 × 10-12 M. This methodology was successfully applied to the quantification of the herbicide in honey. From Raman experiments with and without silver nanoparticles, it was possible to state that the hemin is the species responsible for the absorption in the absence or the presence of the herbicide via vinyl groups. Likewise, when the glyphosate concentration increases, a subtle increase occurs in the planar orientation of the vinyl group at position 2 in the porphyrin ring of hemin over the silver surface, favoring the reduction of the molecule. The total Raman signal of the hemin-glyphosate incubated solutions includes a maximized electromagnetic contribution by the use of the appropriate laser excitation, and chemical contributions related to charge transfer between silver and hemin, and from resonance properties of Raman scattering of hemin. Incubation of the reporter molecule with the analyte before the conjugation with the SERS substrate has not been explored before and could be extrapolated to other reporter-analyte systems that depend on a binding equilibrium process. FULL TEXT

Klingelhöfer et al., 2020

Klingelhöfer, D., Braun, M., Brüggmann, D., & Groneberg, D. A.; “Glyphosate: How do ongoing controversies, market characteristics, and funding influence the global research landscape?;” Science of The Total Environment, 2020, 765, 144271; DOI: 10.1016/j.scitotenv.2020.144271.


Glyphosate is a systemic broad-spectrum herbicide that is by now the most extensively used herbicide in the world and has been the source for a still heated controversy about its harmful effects on human health and the environment. The different weighting of scientific studies has led to different attitudes in most countries towards appropriate handling and their regulatory authorities. Therefore, an in-depth analysis of the global research landscape on glyphosate is needed to provide the background for further decisions regarding appropriate and careful use, taking into account the different regional conditions. The present study is based on established bibliometric methodological tools and is extended by glyphosate-specific parameters. Chronological and geographical patterns are revealed to determine the incentives and intentions of international scientific efforts. Research output grew in line with the exponential growth in consumption, with the field of research becoming increasingly multidisciplinary and shifting towards environmental and medical disciplines. The countries with the highest herbicide use are also the leading countries in glyphosate research: USA, Brazil, Canada, China and Argentina. The link between publication output and market parameters is as evident as the association with national grants. The research interest of the manufacturing company Monsanto could be shown as the second largest publishing institution behind the US Department of Agriculture, which interest is underscored by its position among the otherwise government-funded organizations. Developing countries are generally underrepresented in glyphosate research, although the use of glyphosate is increasing dramatically. In conclusion, the incentives are strongly linked to market and agricultural interests, with the scientific infrastructure of the countries forming the basis for financing and conducting research. The existing international network is important and needs to be expanded and strengthened by including the lower economies in order to take into account all regional and social needs and aspects of glyphosate use.

Guan et al., 2021

Guan, J., Yang, J., Zhang, Y., Zhang, X., Deng, H., Xu, J., Wang, J., & Yuan, M. S.; “Employing a fluorescent and colorimetric picolyl-functionalized rhodamine for the detection of glyphosate pesticide;” Talanta, 2021, 224, 121834; DOI: 10.1016/j.talanta.2020.121834.


The ongoing poisoning of agricultural products has pushed the security problem to become an important issue. Among them, exceeding the standard rate of pesticide residues is the main factor influencing the quality and security of agricultural products. Monitoring pesticide residues and developing simple, yet ultrasensitive detection systems for pesticide residues are urgently needed. In this study, we successfully developed a novel rhodamine derivative as fluorescent and colorimetric chemosensor R-G for the rapid, selective and ultrasensitive detection of glyphosate pesticide residue in aqueous solution. Through a Cu(2+)-indicator displacement strategy, glyphosate can displace an indicator (R-G) from a Cu(2+)-indicator complex due to its strong affinity to bind with Cu(2+) to give a turn-on fluorescence and distinct color change. Moreover, a test strip was also fabricated to achieve a facile detection of glyphosate pesticide. To demonstrate the possibility of practical applications, glyphosate was detected on the surface of cabbage and in a spiked soil sample. The detection limit of 4.1 nM and the response time of 2 min indicate that the method is enough sensitive and rapid to detect the glyphosate residue at or below levels that pose a health risk. FULL TEXT

Abdel-Halim and Osman, 2020

Abdel-Halim, K. Y., & Osman, S. R.; “Cytotoxicity and Oxidative Stress Responses of Imidacloprid and Glyphosate in Human Prostate Epithelial WPM-Y.1 Cell Line;” Journal of Toxicology, 2020, 2020, 4364650; DOI: 10.1155/2020/4364650.


Insecticide imidacloprid and herbicide glyphosate have a broad spectrum of applicable use in the agricultural sector of Egypt. Their ability to induce in vitro cytotoxic and oxidative stress on normal human cells (prostate epithelial WPM-Y.1 cell line) was evaluated with the methyl tetrazolium test (MTT) and histopathological investigation. Cell viability was evaluated with an MTT test for 24 h. The median inhibition concentration (IC50) values were 0.023 and 0.025 mM for imidacloprid and glyphosate, respectively. Sublethal concentrations: 1/10 and 1/50 of IC50 and IC50 levels significantly induced an increase in the lactate dehydrogenase (LDH) activity and malondialdehyde (MDA) level compared with the untreated cells. Rapid decrease in the glutathione (GSH) content and glutathione-S-transferase (GST) activity was induced. Significant increases were recorded in activities of catalase (CAT), glutathione peroxidase (GPx), and glutathione reductase (GR), respectively, compared with the control group. Transmission electron microscopic (TEM) investigation showed significant defects in the cells following pesticide treatments for 24 h. Therefore, it is concluded that imidacloprid and glyphosate are very toxic in vitro assays and able to induce apoptotic effects as well as oxidative stress. So, these findings provide a scenario of multibiomarkers to achieve the imposed risks of pesticides at low doses. FULL TEXT

Arregui et al., 2004

Arregui, M. C., Lenardon, A., Sanchez, D., Maitre, M. I., Scotta, R., & Enrique, S.; “Monitoring glyphosate residues in transgenic glyphosate-resistant soybean;” Pest Management Science, 2004, 60(2), 163-166; DOI: 10.1002/ps.775.


The availability of Roundup Ready (RR) varieties of soybean has increased the use of glyphosate for weed control in Argentina. Glyphosate [(N-phosphonomethyl)glycine] is employed for the eradication of previous crop vegetation and for weed control during the soybean growing cycle. Its action is effective, and low environmental impact has been reported so far. No residues have been observed in soil or water, either of glyphosate or its metabolite, AMPA (aminomethylphosphonic acid). The objective of this work was to monitor glyphosate and AMPA residues in soybean plants and grains in field crops in Santa Fe Province, Argentina. Five sites were monitored in 1997, 1998 and 1999. Individual soybean plants were sampled from emergence to harvest, dried and ground. Analysis consisted in residue extraction with organic solvents and buffers, agitation, centrifugation, clean-up and HPLC with UV detection. In soybean leaves and stems, glyphosate residues ranged from 1.9 to 4.4 mg kg(-1) and from 0.1 to 1.8 mg kg(-1) in grains. Higher concentrations were detected when glyphosate was sprayed several times during the crop cycle, and when treatments approached the flowering stage. AMPA residues were also detected in leaves and in grains, indicating metabolism of the herbicide.

Kougias et al., 2020

Kougias, D. G., Miller, E., McEwen, A., Reamer, H., Kovochich, M., & Pierce, J.; “Risk Assessment of Glyphosate Exposures from Pilot Study with Simulated Heavy Residential Consumer Application of Roundup((R)) using a Margin of Safety (MOS) Approach;” Risk Analysis, 2020; DOI: 10.1111/risa.13646.


Due to the widespread application of glyphosate, a nonselective herbicide, to a variety of resistant food crops, the general population is exposed to glyphosate through dietary intake. Despite this, dietary exposures to glyphosate are considered low in comparison to application-related exposures. Although previous studies have evaluated exposure to horticultural and agricultural workers, to date only one study, which we recently conducted, has characterized exposure to glyphosate in consumers following heavy residential application of a glyphosate-containing herbicide in a residential yard and garden setting. In this previous study, we demonstrated that urinary glyphosate concentrations in these applicators were similar to or in some circumstances greater than those in occupational applicators, likely due to the nature of the simulation study, which ensured a heavy application protocol. However, it is unknown whether these urinary glyphosate concentrations in consumer applicators correspond to internal doses that may be of concern. Therefore, the purpose of this study is to provide a comprehensive risk assessment of glyphosate exposure in consumer applicators using a margin of safety approach. Here, we incorporated data collected from multiple spot urine samples across time from our previous study that assessed consumer exposure to glyphosate from Roundup((R)) application. Estimated internal doses, even with the use of conservative assumptions across unique approaches, were below internal doses estimated from established health-based guidance values. Overall, this study demonstrates that glyphosate exposure from even heavy consumer application of a commercially available glyphosate-containing herbicide does not appear to be a health concern. FULL TEXT

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