Romano et al., 2010
Romano, R. M., Romano, M. A., Bernardi, M. M., Furtado, P. V., & Oliveira, C. A.; “Prepubertal exposure to commercial formulation of the herbicide glyphosate alters testosterone levels and testicular morphology;” Archives of Toxicology, 2010, 84(4), 309-317; DOI: 10.1007/s00204-009-0494-z.
ABSTRACT:
Glyphosate is a herbicide widely used to kill weeds both in agricultural and non-agricultural landscapes. Its reproductive toxicity is related to the inhibition of a StAR protein and an aromatase enzyme, which causes an in vitro reduction in testosterone and estradiol synthesis. Studies in vivo about this herbicide effects in prepubertal Wistar rats reproductive development were not performed at this moment. Evaluations included the progression of puberty, body development, the hormonal production of testosterone, estradiol and corticosterone, and the morphology of the testis. Results showed that the herbicide (1) significantly changed the progression of puberty in a dose-dependent manner; (2) reduced the testosterone production, in semineferous tubules’ morphology, decreased significantly the epithelium height (P < 0.001; control = 85.8 +/- 2.8 microm; 5 mg/kg = 71.9 +/- 5.3 microm; 50 mg/kg = 69.1 +/- 1.7 microm; 250 mg/kg = 65.2 +/- 1.3 microm) and increased the luminal diameter (P < 0.01; control = 94.0 +/- 5.7 microm; 5 mg/kg = 116.6 +/- 6.6 microm; 50 mg/kg = 114.3 +/- 3.1 microm; 250 mg/kg = 130.3 +/- 4.8 microm); (4) no difference in tubular diameter was observed; and (5) relative to the controls, no differences in serum corticosterone or estradiol levels were detected, but the concentrations of testosterone serum were lower in all treated groups (P < 0.001; control = 154.5 +/- 12.9 ng/dL; 5 mg/kg = 108.6 +/- 19.6 ng/dL; 50 mg/dL = 84.5 +/- 12.2 ng/dL; 250 mg/kg = 76.9 +/- 14.2 ng/dL). These results suggest that commercial formulation of glyphosate is a potent endocrine disruptor in vivo, causing disturbances in the reproductive development of rats when the exposure was performed during the puberty period. FULL TEXT
Guerrero Schimpf et al., 2017
Guerrero Schimpf, M., Milesi, M. M., Ingaramo, P. I., Luque, E. H., & Varayoud, J.; “Neonatal exposure to a glyphosate based herbicide alters the development of the rat uterus;” Toxicology, 2017, 376, 2-14; DOI: 10.1016/j.tox.2016.06.004.
ABSTRACT:
Glyphosate-based herbicides (GBHs) are extensively used to control weeds on both cropland and non-cropland areas. No reports are available regarding the effects of GBHs exposure on uterine development. We evaluated if neonatal exposure to a GBH affects uterine morphology, proliferation and expression of proteins that regulate uterine organogenetic differentiation in rats. Female Wistar pups received saline solution (control, C) or a commercial formulation of glyphosate (GBH, 2mg/kg) by sc injection every 48h from postnatal day (PND) 1 to PND7. Rats were sacrificed on PND8 (neonatal period) and PND21 (prepubertal period) to evaluate acute and short-term effects, respectively. The uterine morphology was evaluated in hematoxylin and eosin stained sections. The epithelial and stromal immunophenotypes were established by assessing the expression of luminal epithelial protein (cytokeratin 8; CK8), basal epithelial proteins (p63 and pan cytokeratin CK1, 5, 10 and 14); and vimentin by immunohistochemistry (IHC). To investigate changes on proteins that regulate uterine organogenetic differentiation we evaluated the expression of estrogen receptor alpha (ERalpha), progesterone receptor (PR), Hoxa10 and Wnt7a by IHC. The GBH-exposed uteri showed morphological changes, characterized by an increase in the incidence of luminal epithelial hyperplasia (LEH) and an increase in the stromal and myometrial thickness. The epithelial cells showed a positive immunostaining for CK8, while the stromal cells for vimentin. GBH treatment increased cell proliferation in the luminal and stromal compartment on PND8, without changes on PND21. GBH treatment also altered the expression of proteins involved in uterine organogenetic differentiation. PR and Hoxa10 were deregulated both immediately and two weeks after the exposure. ERalpha was induced in the stromal compartment on PND8, and was downregulated in the luminal epithelial cells of gyphosate-exposed animals on PND21. GBH treatment also increased the expression of Wnt7a in the stromal and glandular epithelial cells on PND21. Neonatal exposure to GBH disrupts the postnatal uterine development at the neonatal and prepubertal period. All these changes may alter the functional differentiation of the uterus, affecting the female fertility and/or promoting the development of neoplasias. FULL TEXT
Caiati et al., 2019
Caiati, C., Pollice, P., Favale, S., & Lepera, M. E.; “The Herbicide Glyphosate and Its Apparently Controversial Effect on Human Health: An Updated Clinical Perspective;” Endocrine, Metabolic, and Immune Disorders: Drug Targets, 2019; DOI: 10.2174/1871530319666191015191614.
ABSTRACT:
BACKGROUND: Glyphosate (G) is the most common weed-killer in the world. Every year tons and tons of G are applied on crop fields. G was first introduced in the mid 1970s and since then its usage has gradually increased to reach a peak since 2005. Now G usage is approximately 100 -fold what it was in 1970. Its impact on human health was considered benign at the beginning. But over the years, evidence of a pervasive negative effect of this pesticide on humans has been mounting. Nonetheless, G usage is allowed by government health control agencies (both in the United States and Europe), that rely upon the evidence produced by the G producer. However, the IARC (International Agency for Research on Cancer) in 2015 has stated that G is probable carcinogenic (class 2A), the second highest class in terms of risk.
OBJECTIVE: In this review, we explore the effect of G on human health, focusing in particular on more recent knowledge.
RESULTS: We have attempted to untangle the controversy about the dangers of the product for human beings in view of a very recent development, when the so -called Monsanto Papers, consisting of Emails and memos from Monsanto came to light, revealing a coordinated strategy to manipulate the debate about the safety of glyphosate to the company’s advantage.
CONCLUSIONS: The story of G is a recurrent one (see the tobacco story), that seriously jeopardizes the credibility of the scientific study in the modern era.
Cuhra, 2015
Cuhra, M.; “Glyphosate nontoxicity: the genesis of a scientific fact;” Journal of Biological Physics and Chemistry, 2015, 15(3), 89-96; DOI: 10.4024/08CU15A.jbpc.15.03.
ABSTRACT:
Repetition of a 1978 experiment on the toxicity of glyphosate chemicals in water-flea Daphnia magna showed surprising results. In the 31 years which had passed between the two series of experiments, the toxicity of glyphosate had apparently become 300 times stronger! Further investigation into this enigmatic paradox discloses unfortunate aspects of laboratory researcher cultures as well as fundamental challenges in current regulatory approval of chemicals and the epistemology of risk-assessment. FULL TEXT
Aparicio et al., 2018
Aparicio, Virginia C., Aimar, Silvia, De Gerónimo, Eduardo, Mendez, Mariano J., & Costa, José L.; “Glyphosate and AMPA concentrations in wind-blown material under field conditions;” Land Degradation & Development, 2018, 29(5), 1317-1326; DOI: 10.1002/ldr.2920.
ABSTRACT:
Agricultural intensification in fragile arid and semiarid environments has led to an increase in soil degradation, mainly through wind erosion. Argentina is an agricultural and cattle‐farming country, which has increased its productivity in the last few decades, widening the boundaries of farm land and the use of herbicides to control weeds. Glyphosate, which accounts for 65% of the Argentinian pesticides market, is strongly retained in soil. The World Health Organization concluded that there was evidence to classify glyphosate as ‘probably carcinogenic to humans.’ In this context, the objective of this study was to determine the presence and concentration of glyphosate and aminomethylphosphonic acid (AMPA) in wind‐blown material in 3 areas in Argentine semiarid regions (Chaco, La Pampa, and San Luis). In 1‐ha2 plots, left uncovered and levelled, the wind‐blown material was collected at heights of 13.5, 50, and 150 cm during 18 erosion events. The wind‐blown material carried by the wind at a height of 150 cm had concentrations of 247 and 218 μgkg−1 of glyphosate and AMPA, respectively. This material was enriched 60 times in glyphosate and 3 times in AMPA as compared with the original soil. This shows that the eroded material can, potentially, have a negative impact on the ecosystem and also on human health, depending on the proportion of this material released into the atmosphere in suspension as particulate matter. This study is, to our knowledge, the first to report concentrations of glyphosate and AMPA in wind‐blown material under field conditions. FULL TEXT
Pandey and Rudraiah, 2015
Pandey, Aparamita, & Rudraiah, Medhamurthy; “Analysis of endocrine disruption effect of Roundup ® in adrenal gland of male rats;” Toxicology Reports, 2015, 2, 1075-1085; DOI: 10.1016/j.toxrep.2015.07.021.
ABSTRACT:
The effect of Roundup((R)) on adrenal gland steroidogenesis and signaling pathway associated with steroid production was investigated. Doses of 10, 50, 100 and 250 mg/kg bw/d Roundup((R)) were administered for two weeks to adult male rats. The 10 mg/kg bw/d dose which reduced circulatory corticosterone levels, but did not change food consumption and body weight, was selected for further study. The expression of cholesterol receptor (low density lipoprotein receptor), de novo cholesterol synthesis enzyme (3-hydroxy-3-methylglutaryl-coenzyme A synthase), hormone-sensitive lipase, steroidogenic acute regulatory protein (StAR) mRNA and phosphorylated form was decreased. Adrenocorticotropic hormone receptor (ACTH), melanocortin-2 receptor, expression was not changed but circulatory ACTH levels and adrenal cortex protein kinase A (PKA) activity were reduced. Surprisingly, exogenous ACTH treatment rescued steroidogenesis in Roundup((R))-treated animals. Apoptosis was evident at 250 mg/kg bw/d, but not at 10 mg/kg bw/d dose. These results suggest that Roundup((R)) may be inhibitory to hypothalamic-pituitary axis leading to reduction in cyclic adenosine monophosphate (cAMP)/PKA pathway, StAR phosphorylation and corticosterone synthesis in the adrenal tissue. FULL TEXT
Miersma et al., 2003
Miersma, Nick A., Pepper, Christopher B., & Anderson, Todd A.; “Organochlorine pesticides in elementary school yards along the Texas–Mexico border;” Environmental Pollution, 2003, 126(1), 65-71; DOI: 10.1016/s0269-7491(03)00126-x.
ABSTRACT:
A reconnaissance study was undertaken to determine potential contaminant exposures to children through soil from elementary school playgrounds. Soil samples were collected from areas along the Texas–Mexico border, inland areas (soils from elementary school yards in cities/towns within the state of Texas), and three National Parks (one on the border, one in Tennessee, and one in Washington). The present study focused on organochlorine (OC) pesticides as the potential contaminants of concern because of their historical (and possibly current) use, and their importance as persistent organic pollutants (POPs). DDE and heptachlor were the most frequently detected OCs (69 and 63%, respectively), although heptachlor concentrations in soil never exceeded 5 ppb. Relatively higher concentrations of DDE were observed in agricultural areas along the border (50–60 ppb in soils from McAllen, Palmview, and San Benito) than in other soils. However, a school yard in Lubbock, TX had the highest OC concentration observed (70 ppb dieldrin). These results may be due to historical agriculture activity prior to the banning of OC pesticides such as DDT in the early 1970s, as well as the more recent use of DDT in Central and South America for malaria control. FULL TEXT
Gilden et al., 2012
Gilden, R., Friedmann, E., Sattler, B., Squibb, K., & McPhaul, K.; “Potential health effects related to pesticide use on athletic fields;” Public Health Nursing, 2012, 29(3), 198-207; DOI: 10.1111/j.1525-1446.2012.01016.x.
ABSTRACT:
OBJECTIVES: Children come in contact with athletic fields on a daily basis. How these fields are maintained may have an impact on children’s potential exposure to pesticides and associated health effects.
DESIGN AND SAMPLE: This is a cross-sectional, descriptive study that utilized a survey to assess playing field maintenance practices regarding the use of pesticides. Athletic fields (N = 101) in Maryland were stratified by population density and randomly selected. MEASURES: A survey was administered to field managers (n = 33) to assess maintenance practices, including the use of pesticides. Analysis included descriptive statistics and generalized estimating equations.
RESULTS: Managers of 66 fields (65.3%) reported applying pesticides, mainly herbicides (57.4%). Managers of urban and suburban fields were less likely to apply pesticides than managers of rural fields. Combined cultivation practice was also a significant predictor of increased pesticide use.
CONCLUSIONS: The use of pesticides on athletic fields presents many possible health hazards. Results indicate that there is a significant risk of exposure to pesticide for children engaged in sports activities. Given that children are also often concurrently exposed to pesticides as food residues and from home pest management, we need to examine opportunities to reduce their exposures. Both policy and practice questions are raised. FULL TEXT
Alarcon et al., 2005
Alarcon, W. A., Calvert, G. M., Blondell, J. M., Mehler, L. N., Sievert, J., Propeck, M., Tibbetts, D. S., Becker, A., Lackovic, M., Soileau, S. B., Das, R., Beckman, J., Male, D. P., Thomsen, C. L., & Stanbury, M.; “Acute illnesses associated with pesticide exposure at schools;” JAMA, 2005, 294(4), 455-465; DOI: 10.1001/jama.294.4.455.
ABSTRACT:
CONTEXT: Pesticides continue to be used on school property, and some schools are at risk of pesticide drift exposure from neighboring farms, which leads to pesticide exposure among students and school employees. However, information on the magnitude of illnesses and risk factors associated with these pesticide exposures is not available.
OBJECTIVE: To estimate the magnitude of and associated risk factors for pesticide related illnesses at schools.
DESIGN, SETTING AND PARTICIPANTS: Analysis of surveillance data from 1998 to 2002 of 2593 persons with acute pesticide-related illnesses associated with exposure at schools. Nationwide information on pesticide-related illnesses is routinely collected by 3 national pesticide surveillance systems: the National Institute for Occupational Safety and Health’s Sentinel Event Notification System for Occupational Risks pesticides program, the California Department of Pesticide Regulation, and the Toxic Exposure Surveillance System.
MAIN OUTCOME MEASURES: Incidence rates and severity of acute pesticide-related illnesses.
RESULTS: Incidence rates for 1998-2002 were 7.4 cases per million children and 27.3 cases per million school employee full-time equivalents. The incidence rates among children increased significantly from 1998 to 2002. Illness of high severity was found in 3 cases (0.1%), moderate severity in 275 cases (11%), and low severity in 2315 cases (89%). Most illnesses were associated with insecticides (n=895, 35%), disinfectants (n=830, 32%), repellents (n=335, 13%), or herbicides (n=279, 11%). Among 406 cases with detailed information on the source of pesticide exposure, 281 (69%) were associated with pesticides used at schools and 125 (31%) were associated with pesticide drift exposure from farmland.
CONCLUSIONS: Pesticide exposure at schools produces acute illnesses among school employees and students. To prevent pesticide-related illnesses at schools, implementation of integrated pest management programs in schools, practices to reduce pesticide drift, and adoption of pesticide spray buffer zones around schools are recommended.
Reynolds et al., 1994
Reynolds, P. M., Reif, J. S., Ramsdell, H. S., & Tessari, J. D.; “Canine exposure to herbicide-treated lawns and urinary excretion of 2,4-dichlorophenoxyacetic acid;” Cancer Epidemiology, Biomarkers, & Prevention, 1994, 3(3), 233-237.
ABSTRACT:
A recent study by Hayes et al. (J. Natl. Cancer. Inst., 83: 1226-1231, 1991) found an increased risk of malignant lymphoma associated with exposure to 2,4-dichlorophenoxyacetic acid (2,4-D) in pet dogs. We conducted a study to determine the extent to which dogs absorb and excrete 2,4-D in urine after contact with treated lawns under natural conditions. Among 44 dogs potentially exposed to 2,4-D-treated lawns an average of 10.9 days after application, 2,4-D concentrations greater than or equal to 10.0 micrograms/l were found in 33 dogs (75%) and concentrations of > or = 50 micrograms/l were found in 17 (39%). Among 15 dogs with no known exposure to a 2,4-D-treated lawn in the previous 42 days, 4 (27%) had evidence of 2,4-D in urine, 1 at a concentration of > or = 50 micrograms/l. The odds ratio for the association between exposure to a 2,4-D-treated lawn and the detection of > or = 50 micrograms/l 2,4-D in urine was 8.8 (95% confidence interval, 1.4-56.2). Dogs exposed to lawns treated within 7 days before urine collection were more than 50 times as likely to have 2,4-D at concentrations > or = 50 micrograms/l than dogs with exposure to a lawn treated more than 1 week previously (odds ratio = 56.0; 95% confidence interval, 10.0-312.2). The highest mean concentration of 2,4-D in urine (21.3 mg/l) was found in dogs sampled within 2 days after application of the herbicide.(ABSTRACT TRUNCATED AT 250 WORDS) FULL TEXT